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Skin irritation (finished products)
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In vitro Skin Irritation assay for finished products on SkinEthicTM RHE or EpiSkinLM models
DESCRIPTION
Our epidermal models are suitable to assess finished products irritation potential.
MODELS
PROTOCOL PRINCIPLE
Both EpiskinSM and SkinEthicTM RHE models can be used for in vitro skin irritation assessment but they should be used with their respective protocol.
Detailed assay procedure
SkinEthicTM skin irritation test method
Click here to download the full detailed protocol
NB: please note that this protocol is currently under revision in order to meet OECD TG439 version 2015; website news will take you inform of new protocol version availability.
Do not hesitate to get information on our training sessions or to contact our technical support.
REFERENCES
Effects of topically applied acitretin in reconstructed human epidermis and the rhino mouse. A. Hsia, M. J. Johnston, R. J. Houlden, W. H. Chern, H. E.J. Hofland. Journal of investigative Dermatology, 128: 125-130, 2008.
Using tissue engineered skin to evaluate the irritation potential of skin care products. B. De Wever, V. Charbonnier. Cosmetics and Toiletries Manufacture Worldwide, 117 : 28-36, 2002.
Use of a serum-free reconstituted epidermis as a skin pharmacological model. O. Doucet, C. Robert, L. Zastrow. Toxicology In Vitro, 10: 305-313, 1996.
Reconstituted epidermis for testing. M. Rosdy. Cosmetics and Toiletries Manufacture Worldwide, 223-226, 1994.
In vitro evaluation of the permeation through reconstructed human epidermis of essentials oils from cosmetic formulations. S. Gabbanini, E. Lucchi, M. Carli, E. Berlini, A. Minghetti, L. Valgimigli. Journal of Pharmaceutical and Biomediacl Analysis, 50: 370-376, 2009.
Lemon (Citrus lemon, Burm.f.) essential oil enhances the trans-epidermal release of lipid-(A, E) and water-(B6, C) soluble vitamins from topical emulsions in reconstructed human epidermis. L. Valgimigli, S. Gabbanini, E. Berlini, E. Lucchi, C. Beltramini, Y. L. Bertarelli. Internation Journal of Cosmetic Science, 34 : 347-356, 2012.
Analysis of in vitro release through reconstructed human epidermis and synthetic membranes of multi-vitamins from cosmetic formulations. S. Gabbanini, R. Matera, C. Beltramini, A. Minghetti, L. Valgimigli. Journal of Pharmaceutical and Biomedical Analysis, 52: 461-467, 2010.