Similarly to all other non-validated tests, users are completely free to develop their own protocol and thus to get test perfectly adapted to their specific needs. This includes thus a multitude of possibility regarding protocol steps (pre-incubation, application, rinsing…) and used endpoints (biochemistry, molecular biology, OMICS, cell imaging...). Moreover the main advantage is the possibility to use well-known references as positive and negative control that may be different from regulatory toxicological ones.
Example of applications:
- Safety assays:
- profiling of potential local tolerance issues from developed compounds or products on the bladder.
- Efficacy assays:
- Evaluating drugs activity effects on urinary bladder cancer.