1992
Biochem Biophys Res Commun 1992 ;184 (1):283-91
Cellife Biotecnologie s.r.l., Universita di Genova, Italy.
Protein kinase C mRNA levels and activity in reconstituted normal human epidermis: relationships to cell differentiation
Although keratinocytes are a major target of phorbol ester actions, the activity and the expression of the eight cloned protein kinase C (PKC) isoenzymes have not been studied in detail in human epidermis. Starting from normal human keratinocytes, we reconstituted in culture a multilayered epithelial tissue which presents many hystological, biochemical, and molecular features of the authentic epidermis and we used it as a model to investigate the PKC activity and mRNA levels. We found that i) PKC activity is higher in differentiated than in non-differentiated cells;